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10.1016/j.theriogenology.2020.06.029 172 Shabajee-AlibayP.BonnaudA.MalpauxB.DelagrangeP.AudinotV.YousS.et al (2022)

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After three washes with TBST buffer, the membranes were incubated at 4 C for 4 h in TBST containing the purified His-tag protein at a final concentration of 35 g/mL