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After sample application, the column was eluted with a linear gradient from 0 to 40% ACN in 0.1% trifluoroacetic acid at a flow rate of 25 mL/min for 30 min, and wavelength 215 nm of the effluent was monitored

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Depletion of total GSH also directly impacts on E GSH and thus mutants with defects in GSH biosynthesis may cause constitutive activation or inactivation of respective signaling pathways