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(2) molecular dynamics simulated annealing restraining FOXO4 FH (without flexible regions) harmonically to its template structure (using a non-crystallographic force constant of 10,000 kcal mol 1 2 ), with additional dihedral angle restraints from 1 H, 15 N, and 13 C chemical shifts using TALOS-N 56 , hydrogen bond restraints (region p53 residues 48-55

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Fluorescence polarization (FP) Fluorescence polarization competition (FAK-paxillin) assay was performed using FAK FAT protein and TAMRA-LD2 L10D peptide 52

doi: 10.1073/pnas.1403438111 97 TanLGurbaniDWeisbergELHunterJCLiLJonesDSet al