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Sequence: Gly-His-Lys (complexed with Cu2+) Molecular Formula: C14H22CuN6O4 Molecular Weight: 401.91 g/mol CAS Number: 89030-95-5 PubChem CID: 73587 Synonyms: Copper Tripeptide-1, GHK Copper, Prezatide Copper, Glycyl-L-histidyl-L-lysine Copper Complex Purity: 99% (HPLC) Presentation: Vial, deionized water, spray bottle Shelf Life: 36 months from date of manufacture when stored properly Quality Verification: Third-party tested by certified laboratory Manufacturing Standard: USA-made, pharmaceutical-grade, GMP compliant Intended Application: In vitro research applications only At Limitless Biotech, we provide comprehensive Certificates of Analysis (COAs) for all of our research-only peptides to ensure transparency, purity, and quality in every batch

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EVs were then purified using size-exclusion chromatography (qEV Original, Izon Science, New Zealand) using PBS pH 7.4 as eluant