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The lack of evidence-based best-practice methodology hampers reproducibility (preclinical or clinical), and translation (between preclinical and clinical work)

Previous reports have indicated that indomethacin is effective at inhibiting NO production by LPS-stimulated microglia in vitro [60]

This simple, relaxing procedure typically takes 30 to 60 minutes

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DAC is believed to tether the peptide to albumin in the media, which may increase exposure time in cell culture and further reduce proteolysis, potentially changing the timing and duration of GHRH-receptor binding