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It was discovered 130 years ago in baker's yeast (Saccharomyces cerevisiae)

MIG-6L::mNG quantification For MIG-6L::mNG fluorescence quantifications, images were processed with Imaris as above, except that 3D objects were generated based on the green channel to include the whole gonad basement membrane

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Portion into small, labeled aliquots to avoid repeated freezethaw cycles, and keep at 2-8C for short-term storage