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Molecular docking identifies 1, 8-Cineole (Eucalyptol) as a novel PPAR agonist that alleviates colon inflammation

These results reveal the dynamic interplay between the structural states of the CRL4 DET1-COP1 E3 ligase complex and its substrate specific activation mechanism, offering mechanistic insights into ubiquitination regulation and a basis for future studies on E3 ligase dynamics

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Biological replicates, isotope-labeled internal standards, and repeated QC checks are used to separate true biological differences from analytical noise, enabling more reliable biological interpretation