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Rectal tumour biopsies ( n =10) were collected from consenting patients undergoing diagnostic endoscopy, a baseline measurement of metabolic rate was recorded, biopsies were treated for 24 h with metformin (10 mM) or H 2 O vehicle control and metabolism was assessed at 24 h post treatment

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Organoids have been successfully developed from EC primary tumor samples of a spectrum of clinical types, ranging from hyperplasia to low- and high-grade cancer [64, 69]

They are responsible for the reabsorption (deactivation) of the neurotransmitters