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Indeed, the determination of the perinuclear mitochondrial CMFDA staining after co-localization with mitotracker red (as described previously [24]) (figure 4C) in 3T3 fibroblasts at 24 hours of culture show that BSO treated cells maintained a high mitochondrial CMFDA staining but cytosolic fluorescence was indeed decreased by BSO

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Dermoscopy is helpful in both instances and where the pigment is heterogeneous in both the longitudinal and transverse axes (Figure 7), the likelihood of melanoma is greater [31]

These factors necessitated the use of nonparametric tests to ensure appropriate statistical inference