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ACSF was composed of 125 mM NaCl, 25 mM glucose, 25 mM NaHCO 3 , 2.5 mM KCl, 2 mM CaCl 2 , 1.25 mM NaH 2 PO 4 and 1 mM MgCl 2 that was saturated with 95% oxygen and 5% carbon dioxide and maintained at room temperature (2022 C)

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