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To evaluate the influence of diet intervention on plasma L-Cn levels, two male participants fasted overnight (10 h) before performing the short-term L-Cn challenge test, which included a natural source of L-Cn (240 g cooked-beef, a major source of dietary L-Cn, equivalent to an estimated 180 mg L-Cn), and venous blood samples were collected at indicated time points for L-Cn detection