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Bilateral craniotomies were made above the VTA to inject 0.5 l of rAAV5-Syn-FLEX-GCaMP6s-WPRE-SV40 (5 * 10^8 genetic copies, Addgene) virus at coordinates AP -3.2 mm, ML 1.5 mm with a 15 angle and DV -4.8

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Avoid high-fat, high-fiber, and heavily processed foods, which take longer to empty from the stomach and may trigger nausea