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Real-time qPCR Total RNA was isolated with TRIZOL reagent (Invitrogen) and reverse transcription was performed by ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO), according to the manufacturers protocol

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And while that can be true, it also introduces complexity

Claims concerning insomnia, sleep disorders, pain, anxiety, withdrawal, cortisol, growth hormone or neurological function may contribute to medicinal-product classification