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Available from Liberati A, Altman DG, Tetzlaff J et al (2009) The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate healthcare interventions: explanation and elaboration

Barnard K, Sinclair JMA, Lawton J, Young AJ, Holt RIG (2012) Alcohol-associated risks for young adults with type 1 diabetes: a narrative review

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After incubation on ice for 30 min and subsequent centrifugation at 4000 g (worms) or maximum speed (~21,000 g for medium) for 1015 min at 4 C, the supernatants were transferred to pre-chilled microcentrifuge tubes, concentrated via SpeedVac, and resuspended in 50% methanol