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the pure Splotch embryos show severe neural crest cell (NCC) defects with complex phenotypes such as spina bifida, extracerebral malformations, and cardiac outflow tract abnormalities ( Tetralogy of Fallot is the most common form of cyanotic congenital heart disease that accounts for 35% of all congenital heart diseases

Dako A/S, Glostrup, Denmark), HMB-45 (monoclonal, dilution 1:200

Where possible, future studies should endeavor to rule out off-target metabolic effects, either by confirming minimal changes in the synthesis of metabolic off-targets or demonstrating that linked nitrogen metabolism pathways are not required for observed effects

Increased melanin does not eliminate ultraviolet DNA damage or replace established sun protection

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