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Modern approaches leverage advanced technologies, including supercritical fluid extraction (SFE) using carbon dioxide, which offers a greener alternative by minimizing solvent residues and preserving delicate compounds

In a typical encapsulation experiment, PLGA nanoparticles exhibited a hydrodynamic diameter of 170 30 nm (z-average, PDI = 0.162) as measured using dynamic light scattering with anionic effective surface charge (20.5 5.3 mV) due to the terminal carboxylate groups on the PLGA polymers

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