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Upon recovery and expansion, a number of clonal cell lines were generated and initially screened for complete 2 -adrenergic receptor knockout by qPCR followed by additional functional validation via insulin secretion and radioligand binding assays

Seiler, Sarah E., Koves, Timothy R., Gooding, Jessica R., Wong, Kari E., Stevens, Robert D., Ilkayeva, Olga R., Wittmann, April H., DeBalsi, Karen L., Davies, Michael N., Lindeboom, L., Schrauwen, P., Schrauwen-Hinderling, Vera B

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