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Injection technique, including proper needle placement and consistent site rotation, matters more for reliable dosing than choosing one site over another
In addition, intra-VTA application of exendin-4 suppresses high-fat food intake, which is consistent with the results of chemogenetic activation of endogenously released GLP-1 nerve terminals in the VTA (Wang et al., 2015)
5c-d), there was no change in the beneficial effect on glycaemia of Ex4 injection into the OB indicating that PNS activity was not implicated in the beneficial effects of Ex4 administration in the OB
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