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METHODS ANIMAL MODEL AND ASSOCIATED OVERSIGHT: All steps of our protocol were designed and carried out under supervision by the Institutional Animal Care and Use Committee at Rhode Island Hospital and veterinary staff employed by Brown University (protocol #505821)

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Common causes include: Pernicious anaemia Malabsorption disorders (e.g., coeliac disease, Crohns) Chronic alcohol abuse Long-term use of antacids or metformin Vegan or vegetarian diet without supplementation 3

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These methods aim to stimulate the vagus or phrenic nerves, alter diaphragmatic contraction patterns, or increase carbon dioxide levels in the blood