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Two Cys are placed at both ends of the linker (C terminus of VL and N terminus of VH) and two in VL-VH domain (L100-H44)

For protein precipitation and metabolite extraction, 25 l of plasma and diluted urine were added to 1.5 ml Eppendorf tubes, along with 225 l of methanol and 10 l of internal standard (IS) of both substances

The activation of hGPX4-C by compound 102 were remarkably reduced for mutant D23A (the compound concentration required to reach 150% activation was higher than 2,500 M)

The positive effects are ascribed to the elevated concentrations of anthocyanins such as cyanidin-3-O-glucoside that are found in purple corn, which exhibit potent antioxidant characteristics