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The intramuscular acidic saline injection model is another commonly used model that uses repeated injections of acidic saline into the gastrocnemius muscle to develop widespread pain 34,35

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The deduced amino acid sequences of AsGGT1 , AsGGT2 , and AsGGT3 contained threonine residues required for autoprocessing and the residues required for GGT activity ( AsGGT1 , AsGGT2 , and AsGGT3 all encode functional GGT proteins