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For immunohistological staining, brainstem sections were blocked with 1% bovine serum albumin (BSA, fraction V) and 1% normal goat serum in 0.2% Triton X-100 in PBS (PBS/T)
As these microclots circulate, they damage the delicate endothelial lining of the blood vessels and disrupt normal cardiovascular function
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PMID:11454572
Italian nurses on rotating NS had higher expression of BMAL1, CLOCK, NPAS2, PER1, PER2, and REV-ERB but lower expression of PER3, CRY1, and CRY2 than DS nurses 206
