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Subsequent research exploring repeat-dose protocols found that spacing administrations 714 days apart allowed clearance of apoptotic debris from the first senolytic wave before introducing a second dose

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This animal model also exhibit a dysregulation of Nrf2-dependent antioxidant responses in the vasculature, which promotes the development of marked endothelial dysfunction and endothelial apoptosis in the presence of increased oxidative stress[281], mimicking the aging phenotype