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He had old liver tissue and young liver tissue, and wanted to know why they made proteins so differently

Petrillo S, DAmico J, La Rosa P, Bertini ES, Piemonte F (2019) Targeting NRF2 for the treatment of Friedreichs ataxia: a comparison among drugs

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This relatively easy and fast technique for detecting protein-protein interactions is compatible with numerous downstream analysis methods such as mass spectrometry, western blot, etc