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The rearing activity in rats was also decreased by the endogenous endocannabinoid anandamide (Fride and Mechoulam, 1993)

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To minimize such unwanted events, we developed a standardized thawing procedure for frozen samples, with a small sample volume (150-200 L): thawing under strict cooling conditions (+0C to +2C) within 12-15 minutes in an ice-water bath, without agitation