Garca-Gimnez JL, Garca-Lpez E, Mena-Moll S, Beltrn-Garca J, Osca-Verdegal R, Nacher-Sendra E, et al
To overcome this limitation, a clinical study combines decitabine and tetrahydrouridine (THU), a cytosine deaminase inhibitor, as a therapeutic strategy for inducing HbF (ClinicalTrials.gov Identifier: NCT01685515)
Each vial undergoes comprehensive USP testing protocols including bacterial endotoxins test (LAL), sterility test (membrane filtration), particulate matter test (light obscuration), and container closure integrity verification
Membranes were blocked during 1 h at room temperature in 1 PBS containing 0.1% Igepal and 5% milk and then incubated overnight at 4C with the following primary antibodies: anti-CBS mouse monoclonal antibody (ab12476, Abcam, 1:1,000) and anti--tubulin mouse monoclonal antibody (T6793, Sigma-Aldrich, 1:10,000)
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