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Fluorescence lifetime imaging microscopy (FLIM) For in vitro lifetime measurements, 0.025 g L -1 NAPstar4.3 diluted in binding buffer, containing 150 M NADP + was added to imaging 8-wells (80806

In vitro cachexia model The TNF--induced C2C12 model is a widely used muscle cell atrophy model that simulates the muscle wasting of cancer cachexia [10, 11, 25]

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It indicates that the positive cell sorting operation was efficient and had great potential to promote the apparent shRNA knockdown efficiency dramatically