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BH4, which is derived from its precursor GTP via three enzymatic reactions catalyzed by GCH1, 6-pyruboyl tetrahydrobiopterin synthase and sepiapterin reductase, exhibits robust antioxidant activity both in vivo and in vitro, directly protecting cells from lipid peroxidation

The resulting extract was deemed 100% CSE and was diluted in normal cell media

And if you want to learn why experts believe 5-10 grams is ideal, check out our article on L-glutamine dosage

g,h,i , Dehydratase activity towards ultrafiltered flavan-3,3,4-triol ( 4 ) and GSH-transferase activity towards CDNB ( 5 ) of variants of Pt GSTF8 ( g ), Ph AN9 ( h ), Vv GST4 ( i ) measured in colorimetric 96-well-plate assays

Metabolism 68:2633