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Insert the applicator tip just inside one nostril and angle it gently toward the outer wall of the nasal passage, away from the nasal septum

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The definitive way to confirm a peptide is laboratory analysis: high-performance liquid chromatography (HPLC) measures purity, mass spectrometry (MS) confirms molecular identity, and a Limulus Amebocyte Lysate (LAL) assay screens for bacterial endotoxins

Reverse transcription of miRNA was performed using the Hairpin-it miRNAs qPCR Quantitation Kit (GenePharma, Shanghai, China), following the manufacturers protocol