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2.5 Reverse transcription and real-time quantitative PCR Total RNA was extracted by using RNAiso Plus reagent (Takara, 9109) following the protocol recommended by the manufacturer

We hypothesize that cells express their antioxidant systems to counteract the oxidative damage caused by resveratrol supplementation

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These combined effects may contribute to improved heart health and reduced risk of cardiovascular complications

Glutathione is often referred to as the "master antioxidant" because of its wide-ranging role in: Neutralizing free radicals Supporting liver detoxification Regenerating other antioxidants (like vitamins C and E) Boosting immune response Improving skin radiance and cellular repair Its naturally produced in the liver using three amino acids: glutamine, glycine, and cysteine