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Materials and methods Chemicals and reagents Cytochrome c, reduced glutathione (GSH), oxidized glutathione (GSSG), 5,5-dithio-bis(2-nitrobenzoic acid), cumene hydroperoxide, dimethylnitrosamine (DMNA), erythromycin, dinitrophenylhydrazine (DNPH), lucigenin, glutathione reductase, thiobarbituric acid, NADH, NADPH, coenzyme Q2, antimycin A, sodium succinate, dodecyl maltoside, Hexokinase assay kits, and ATP Bioluminescent cell assay kits were purchased from Sigma-Aldrich Fine Chemicals (St Louis, MO, United States)
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The cells were collected, washed and ROS was quantified on a NovoCyte flow cytometer, with an excitation max of 640 nm and emission max of 665 nm
Its important to note, however, that these participants took tirzepatide to manage their diabetes rather than exclusively for weight loss
However, their benefits extend beyond blood sugar control, offering promising results for weight management, cardiovascular health, and even cognitive function
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