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For IHC, deparaffinized and rehydrated sections were subjected to antigen retrieval in citrate buffer (pH 6.0) at 95 C for 15 min

For the underlying technique how to add the diluent slowly down the vial wall, why you never shake it, and how to store the solution afterward see the full peptide reconstitution guide and the companion note on how much bacteriostatic water to use

It is advisable to consult a healthcare professional before taking Glowsik L Glutathione Reduced Capsule alongside other medications

Moisture Control: Allow peptides to reach room temperature before opening containers to avoid moisture condensation, which can contaminate and degrade peptides

Afterwards, cell counting kit-8 (CCK-8) solution (Cat #C0005, Topscience, China) was applied to each well, and the plate was subjected to one hour of incubation