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The resulting lysate was separated by ultracentrifugation at 100,000 g for 20 min at 4 C (Beckman Coulter Optima MAX-XP, MLA-80 rotor)

Brain (2018) 141(9):260518

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Vehicle-treated SGm exposed to 15 Gy irradiation exhibited a 3.3-fold increase in the number of H2AX foci per nucleus, indicating a significant increase in double-stranded DNA breaks due to radiation exposure (Fig