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According to the reaction system of SYBR Green analysis, 500 ng of total RNA was needed, and the required RNA volume was calculated as VRNA = 500/C RNA (L)

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We gratefully acknowledge assistance from the UNSW Biological Resource Centre and Tzong-Tyng Hung from the Biological Resource Imaging Laboratory (BRIL) of the Mark Wainwright Analytical Centre, UNSW, which is a member of the National Imaging Facility, and the Bioanalytical Mass Spectrometry Facility of the Mark Wainwright Analytical Centre at UNSW