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All collected samples were diluted to a protein concentration of 1 ng/l, and 5 l of each sample was loaded on a Tris-Glycine gel (Novex Tris-Glycine Mini Protein Gels, 1020%, 1.0 mm, WedgeWell) for Western blot analysis

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coli provides evidence that methylation state of GATC motifs can control the phase-variation of pilus gene whereas the methylation of GATC sites in the promoter region of antigen 43 ( agn43 ) gene leads to transcription initiation 9

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