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The immunoblots were visualized using ECL Western blotting substrate (Pierce Biotechnology) on a Chemidoc XRS imager (Bio Rad) or using Dylight 800-conjugated secondary antibodies (Thermo Fisher Scientific, Rockford, IL) on an Odyssey Imaging Systems (LI-COR Biosciences, Lincoln, NE)

SAG : Animal studies show oral SAG (30 mg/kg for 8 weeks) significantly restores liver GSH, reduces GSSG (oxidized glutathione), and lowers lipid peroxidation in CCl-induced liver injury

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