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The cells were subsequently washed with 250 l lysis buffer, incubated for 15 min with 500 l lysis buffer, and incubated for 10 min in 500 l stimulation buffer (similar to lysis buffer but with 0 or 5 mmol/l ATP and 1.65 or 6.65 mmol/l CaCl 2 ) at 37 C, 5% CO 2

Aldosterone induced by AngII promoting the reabsorption of sodium and water from the kidney, can increase water retention, and norepinephrine secretion via positive feedback from the sympathetic end of the anterior membrane, eventually leading to an increase in BP

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And muscle is your metabolic engine

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