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82 In a randomized controlled trial in 46 elderly subjects with insomnia, 500 mg of elemental magnesium, taken as two divided doses of magnesium oxide daily for eight weeks, increased blood melatonin levels, decreased cortisol levels, and improved self-reported total sleep time, sleep efficiency, and time to sleep onset

After washing, the membranes were incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody