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Vidal A, et al

P2 was suspended in 10 vol of 0.32 M sucrose and 10 ml of the solution layered on a 0.8 and 1.2 M two-step discontinuous gradient, which was centrifuged at 61,000 x g for 2 h to separate the synaptosomes from the mitochondria (which forms the pellet)

doi: 10.1016/j.clindermatol.2011.09.013

Moreover, the results indicated that 0.1 mM melatonin preserved higher adenosine triphosphate levels and prevented the release of cytochrome c into the cytoplasm