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The next mobile phases to be used were methanol (solvent A) and water with the addition of 0.1 % TFA (solvent B), acetonitrile (solvent A) and water with the addition of 0.1 % TFA (solvent B) as well as acetonitrile (solvent A) and water with the addition of 0.04 % HCOOH (solvent B)

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Women with PCOS face significantly increased risks of developing type 2 diabetes mellitus, with studies suggesting up to 50% may develop impaired glucose tolerance or diabetes by age 40

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