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Quantitative real-time PCR (qRT-PCR) was performed using 2 sybr Green qPCR MasterMix (Sevenbio, Beijing) and the ABI StepOnePlus real-time PCR system (Applied Biosystems, United States), with -actin as the internal reference

Control mice received an equivalent volume of saline solution

ASP has also been chemically modified with allantoic acid to generate amphiphilic polymers that self-assemble into nanoparticles through carboxyl-mediated interactions

Researchers dont think so, and the gene expression data backs them up

Placenta-specific methylation of the vitamin D 24-hydroxylase gene: implications for feedback autoregulation of active vitamin D levels at the fetomaternal interface