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Glutamine can be synthesized by cells through GLUL / glutamine synthetase (GS), which catalyzes the condensation reaction between glutamate and ammonia in an ATP-dependent manner to produce glutamine

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To further confirm the findings, pseudotime analysis revealed that clusters expressing high monocyte markers served as root cells, and macrophages differentiated through the intermediate cluster 3 to produce the activated mature innate immunologic cells or the mature alveolar macrophages (Fig