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While ATCUN motifs efficiently complex with Cu(II), they are unable to effectively reduce Cu(II) as they do not bind well to Cu(I) and thus less readily act as redox intermediates 10

269 While ROSALIND is limited to the use of well-characterized apo-repressors, cell extract-based In vitro transcription-translation (Tx-Tl) systems permit the development of biosensors with the whole array of possible aTFs 622,651,652 and orthogonal regulatory elements, including riboswitches (Section 3.4), 653 since both protein- and RNA-based parts make use of the same endogenous RNA polymerase