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Determination of tissue antioxidant capacity Hyperthyroid-induced rats exhibited pronounced disruptions in tissue redox balance, as evidenced by a significant reduction in TAC and a concomitant increase in MDA levels relative to the euthyroid control group (Table 5)

(Bunkelmann and Trelease, 1996), A

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Brain tissue was homogenised with 1:10 (w/v) PBS on ice, then centrifuged at 3,000 rpm for 15 min at 4 C in an Eppendorf centrifuge and the supernatant was collected