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Purification and culturing of human CD4 + T cells and CD8 + T cells CD4 + T cells or CD8 + T cells were purified from PBMCs using magnetic positive selection with biotin human anti-CD4 or anti- CD8 antibodies, respectively, followed by incubation with streptavidin-coupled magnetic microbeads (BioLegend) and positive selection with magnets

The glycemic improvements were equally impressive, with 73.5% of combination participants achieving HbA1c levels at or below 6.5%

All blood samples were drawn at each hospital, as described (14)

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