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While the serum concentration of cobalamin is used diagnostically, the reactions catalyzed by cobalamin dependent enzymes occur in the mitochondria, making it difficult to assess the state of cobalamin availability in the patient
Mol Ther Nucleic Acids 36(2):102543 Acknowledgements The authors thank all participants who were included in this study
Cell lysate proteins (515 g) were fractionated in 415% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels (Bio-Rad Laboratories) and transferred to polyvinylidene difluoride membranes (Trans-Blot Turbo
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