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Dehydrated cells are more vulnerable to ionic disruption

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Preparation of tissue lysates and metabolite analysis Flash-frozen mouse tissues were homogenized in PBS supplemented with protease (cOmplete, Roche) and phosphatase (PhosSTOP, Roche) inhibitors using the IKA Ultra Truemax T8 homogenizer on ice

The cooperation of endocrinologists, nutritionists and other specialists is essential for the comprehensive care of HT patients and the prevention of complications, including metabolic disorders