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The sections were then washed three times in TNT buffer (0.1 M TrisHCl, 0.15 M NaCl, 0.05% Tween20, pH 7.5) for 5 min, and treated with secondary antibodies

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37,41 To fully understand the consequence of preterm birth, it is essential to understand both the effects of reduced gestational age at birth and the effects of the special delivery and clinical care procedures associated with preterm birth, like incubators-rearing, limited maternal contact, specialized care and parenteral/enteral nutrition

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