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In case blood samples were further processed for a scRNA-seq, a density gradient centrifugation was then conducted using Histopaque 1077/1119 (Sigma-Aldrich) following the manufacturers protocol

However, for those with more severe deficiencies, it might take a bit longer or require a series of injections to fully replenish their stores and experience the full benefits

H 2 O 2 stimulates CBS and GCS and inhibits MS and BHMT

To account for potential outliers, an additional eight subjects (2 per arm) results in a total sample size of N = 128

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