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The most effective concentration of HGF (10 ng/ml) was subsequently found to stimulate hippocampal neurons in organotypic hippocampal slice cultures which are more intact preparations similar to Dihexa (Figure 8A and B) further establishing a mechanistic link between Dihexa and HGF/c-Met

The selection of peptide segments can be based on an understanding of the structure and function of the target molecule or obtained through screening large peptide libraries to identify segments with specific binding properties

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The sulfur atom is then incorporated from cysteine